FastQCFastQC Report
Sun 14 Apr 2019
SRR6575689_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575689_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3121506
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA158350.5072871876587776No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC89070.2853430363420733No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC80120.2566709786878513No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC62440.2000316513887848No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG55610.17815118727947343No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC49370.1581608364680382No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44090.1412459242429776No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA42780.13704923200532051No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA41410.132660324856015No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC37720.12083910778963743No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT37590.12042264214773253No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA35990.11529691117044144No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT34320.10994692946289387No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC34280.1098187861884616No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG34040.10904992654186794No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT33010.1057502372252368No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT32160.1030271926435509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22100.062.5336461
GTATCAA90400.054.4943581
ATCAACG107100.045.1882553
TCAACGC109800.044.1626554
CAACGCA110200.044.0016485
TATCAAC114900.042.3018382
AACGCAG115100.042.2917146
ACGCAGA136950.035.372647
CGCAGAG141550.034.223128
GCAGAGT150300.032.3245439
GTACATG169900.032.204631
TACATGG170600.031.8796142
TTAGGTA27850.031.5496254
TAGGTAT28300.031.3795135
ACATGGG172350.031.1610973
CTTAGGT28200.031.1580523
AGGTATA28250.031.1024076
GTCTTAG35900.030.9012661
AAGGGTA15750.030.727525
GGTAATC15350.030.303848
Produced by FastQC (version 0.11.2)