FastQCFastQC Report
Sun 14 Apr 2019
SRR6575692_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575692_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245992
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6320.25691892419265666No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4810.19553481414029728No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4140.1682981560375947No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3720.15122443006276629No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3430.13943542879443233No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3100.12602035838563858No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3090.1256138411005236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3080.12520732381540864No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2730.11097921883638494No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2690.10935314969592506No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2690.10935314969592506No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2690.10935314969592506No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG2610.10610101141500536No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT2610.10610101141500536No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2570.10447494227454551No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2500.10162932127874076No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2490.1012228039936258No Hit
GATCAAAACCAACCCGGTGAGCTCCCTCCCGGCTCCGGCCGGGGGTCGGG2460.10000325213828092No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACTC250.00523680856.384638
GCGTCTA250.00523680856.384639
ATGGGCG558.799034E-751.2587555
GTCGTAA250.0017033637.58975238-39
GTATCAA10850.037.24331
GGTACCT2150.037.1526648
ATGGGTA2700.036.5455935
CATGGGC3100.036.3771824
GTAGGGG1204.1891326E-935.2403956
AGGGGTG1204.1891326E-935.2403958
ACCCTTG959.4306597E-734.6221437
ACTAACT550.002716504734.1725043
GTACATG18400.033.963571
CATGGGT4350.033.4851264
GGGTACC2400.033.2825977
ACATGGG18950.032.481913
TACATGG19450.031.6469022
CGTAAAC300.004164239431.32479540-41
CATGGGG6000.031.3247934
TGGGTAC2750.030.7552536
Produced by FastQC (version 0.11.2)