FastQCFastQC Report
Sun 14 Apr 2019
SRR6575692_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575692_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245992
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6890.28009040944420954No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4810.19553481414029728No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4300.17480243259943412No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4030.16382646590133013No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4020.16341994861621517No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.15285049920322613No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3570.14512667078604183No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3330.13537025594328272No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3290.13374418680282285No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3230.13130508309213307No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3120.12683339295586848No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3050.12398777196006375No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2950.1199225991089141No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2900.1178900126833393No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG2880.11707697811310937No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2880.11707697811310937No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT2790.11341832254707471No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG2700.10975966698104002No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG2660.10813359784058019No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT2570.10447494227454551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10250.041.7888371
AACGCAG10900.037.939546
CCTATGC906.1414175E-736.57264394
GTACATG18250.036.366291
ACATGGG18250.035.7921523
ATGGGTA3050.035.437565
CAACGCA11850.035.2945375
TACATGG18950.034.965982
ATCAACG11950.034.6059383
TCAACGC12000.034.4617464
GCTATAG550.002693189534.2326131
GGCTTAC1107.66413E-834.176947
CATGGGG7300.034.1184164
CATGGGT4900.033.566644
ATGGGGG2450.032.6075945
GGTACCT2600.032.553678
ACGCAGA12950.031.5707867
CGCAGAG13000.031.4685468
TGGGTAC2700.031.3288616
GGGTACC2750.030.7592477
Produced by FastQC (version 0.11.2)