FastQCFastQC Report
Sun 14 Apr 2019
SRR6575695_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575695_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2876083
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA302161.050595549572109No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC206140.7167387032988964No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC203550.7077333999053574No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC129860.45151687207914376No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC127520.4433808064649038No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG108940.378779054707392No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT108560.37745781328285727No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT89650.31170866765667055No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA83880.29164665971044645No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA77730.2702634103396877No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT77050.26789908357999404No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG76940.26751661895710244No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA75880.26383105077287405No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC74450.258859010675283No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT70980.24679399029861102No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG69400.24130040753344043No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC67670.23528528210069044No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT64210.22325503123519036No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA63950.22235102394471928No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT60480.21028600356804722No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT57870.20121116115216423No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG57640.20041146239520904No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT56200.195404652786446No Hit
GTTTGGGGCTGACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACG53490.18598211525884337No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA51370.17861097889038668No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA51250.1781937447563231No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA50780.1765595777312407No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT50660.17614234359717712No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA48390.16824966456114096No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA47690.16581579877910338No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG46750.16254746472893863No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC46180.1605656025921366No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA45590.15851420143299064No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC45400.1578535807207233No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT45140.1569495734302522No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG42170.14662302861217844No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT41410.14398054576310906No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA41380.14387623722959317No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC41040.14269407384974633No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACA40960.14241591776037063No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA39960.13893896664317407No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG39230.1364007923276206No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA38810.13494047285839803No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG38570.1341060045902709No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA38380.13344538387800353No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT38200.13281953267690816No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC37670.13097674858479397No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG37160.12920350351502374No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG36830.12805610964634886No Hit
GGGTATATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT36580.12718687186704972No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG35990.1251354707079038No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC35940.12496162315204394No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA35880.12475300608501215No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACATAGTT33900.11786864287296299No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT33490.1164430929149124No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA33180.11536523806858147No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33160.11529569904623754No Hit
GTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTTAATGT32470.11289660277537192No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC31820.11063658454919416No Hit
CCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTT31810.1106018150380222No Hit
GTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCATAG31230.10858518339004819No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC30780.10702055538730976No Hit
GGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATT30110.10469099813878806No Hit
GTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTG29820.10368268231480107No Hit
GTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCA29450.10239621040143834No Hit
GTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTC29270.10177035920034296No Hit
GATTAATGGGGAATGGCATACTATTATCCTGGCCTCTGACAAAAGAGAAA28790.10010142266408864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13600.062.221051
GTATCAA78500.045.7538261
GTGCAAG21150.045.344391
TCAACGC76750.044.947774
CAACGCA77050.044.833765
ATCAACG76950.044.769873
TATCAAC78400.044.2531362
AACGCAG79750.043.374816
AAGGGTA19450.042.28725
AGGGTAA22050.037.3009536
ACGCAGA93650.036.886727
TGCAAGG22650.036.7374342
GTACATG122300.036.402171
GGTAATC22700.036.2328648
CGCAGAG95800.035.9607668
ACATGGG126650.035.031383
GCAGAGT100450.034.342879
CATGGGG87100.033.7789884
AGGTATA65150.033.184396
CTTAGGT64750.033.0264633
Produced by FastQC (version 0.11.2)