FastQCFastQC Report
Sun 14 Apr 2019
SRR6575696_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575696_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2672834
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA282791.0580155744801212No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC203010.7595308949227674No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC188740.7061418703892572No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC122460.45816537802197965No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC117900.44110483479333173No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG100180.3748081624223577No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT97550.364968419288291No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT84090.31460988598618544No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA77790.29103939863081657No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC72940.2728938647143818No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA71910.26904027709913897No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG71500.26750632474744035No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA71410.2671696034995065No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT68620.25673124481355747No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT67080.2509695701266895No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG63720.23839864353715945No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC62020.2320383532984091No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT59590.22294687960419537No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA58450.21868174379703342No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT58380.21841984949308485No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT58130.21748451269326866No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT56360.21086232815056977No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG55020.2058489229035548No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT53440.19993759432871627No Hit
GTTTGGGGCTGACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACG51560.19290386159409825No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA50440.18871355273092158No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA47570.1779758862690313No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG46750.17490798156563409No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA45270.16937078771072203No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA44200.1653675462075086No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA43700.16349687260787615No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC42210.15792226528097145No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG42130.15762295750503025No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA42090.15747330361705966No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC41920.1568372745931846No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT40960.1532455812818903No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA39420.14748390659502236No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA39020.1459873677153164No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG38730.1449023770275296No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA37680.14097396246830143No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT37610.1407120681643529No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACA37090.13876656762073514No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC36650.13712037485305859No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT36640.13708296138106596No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA36570.1368210670771174No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG36560.13678365360512476No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG35680.13349126806977166No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG35150.13150835405416125No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC34830.13031112295039646No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA34540.12922613226260965No Hit
GGGTATATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT34540.12922613226260965No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA33820.12653236227913892No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG33220.12428755395957998No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC32720.12241688035994752No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC32620.12204274564002104No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT32450.12140671661614601No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACATAGTT31940.11949862954452092No Hit
GGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATT30690.11482194554543977No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30400.11373695485765295No Hit
GTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCATAG30180.11291385847381469No Hit
GTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTTAATGT30030.11235265639392496No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC29960.11209076208997641No Hit
CCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTT29630.11085611751421899No Hit
GTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTC28720.10745149156288793No Hit
ATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAATTCGGAGC28450.10644132781908641No Hit
GATTAATGGGGAATGGCATACTATTATCCTGGCCTCTGACAAAAGAGAAA28210.10554340449126284No Hit
TCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAAT28160.10535633713129959No Hit
ATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTG28050.10494478893938045No Hit
GTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCA27710.10367273089163039No Hit
GTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTG27060.1012408552121082No Hit
GAGGTATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGA26900.10064223966022581No Hit
GGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGC26820.10034293188428461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16500.065.511381
GTATCAA78550.044.215151
GTGCAAG22150.043.4963651
ATCAACG77100.043.3313
TCAACGC77900.042.9463274
CAACGCA78400.042.61255
TATCAAC80450.041.8280872
AACGCAG80900.041.469926
AAGGGTA19250.041.007385
GTACATG114550.036.5557331
TGCAAGG21850.036.1358952
ACGCAGA93600.035.742727
ACATGGG118300.035.3102263
CGCAGAG95150.035.2098548
GGTAATC22300.035.1880468
AGGGTAA23300.034.2828146
CTTAGGT59650.033.556963
GCAGAGT100450.033.351479
TACATGG126100.033.245342
AGGTATA59650.033.2418756
Produced by FastQC (version 0.11.2)