FastQCFastQC Report
Sun 14 Apr 2019
SRR6575696_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575696_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2672834
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA302391.1313459795857133No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC194010.7258587701293833No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC179890.6730309476757629No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC112810.4220613775490734No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC107660.4027934394728591No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT93830.35105060770702556No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG90180.3373946904297087No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT79960.2991581220532214No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA72400.27087353722677876No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC69300.25927536090905756No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG68450.2560952157896824No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT67030.25078250276672626No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC66720.24962268513495414No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA66400.24842545403118937No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT64470.24120465393660812No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA61830.23132749733054875No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG59100.22111361947655558No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG59080.2210387925325703No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA57480.21505263701374647No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT57230.21411730021393022No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT55900.20914130843890794No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT55250.20670943275938572No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT54140.20255653736820167No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG50970.19069646674653196No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA50630.18942440869878188No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT47440.17748951113312686No Hit
GTTTGGGGCTGACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACG46850.17528211628556056No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA46560.17419712559777373No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA45790.17131628825433978No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA45700.17097956700640593No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC45500.17023129756655295No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC45340.16963268201467058No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT43830.1639832477437806No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG42680.15968069846462593No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA41990.15709916889713316No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA41740.15616383209731693No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC40170.15028991699447106No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA40090.14999060921852986No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA38370.1435554920357942No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG38190.14288204953992653No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC37590.14063724122036758No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT36910.13809312512486746No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA36710.13734485568501448No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACA36610.136970720965088No Hit
ATCATAGTCTGTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCA35950.13450143181357316No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT35620.13326678723781574No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA35400.13244369085397747No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG35090.13128387322220533No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA35050.13113421933423475No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC34040.1273554586629772No Hit
GCATACTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATG32860.12294066896784463No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG32590.12193050522404308No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACATAGTT32370.12110740884020482No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT32160.12032172592835919No Hit
GATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACAGAAAAGGC32130.12020948551238124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31980.11964828343249151No Hit
TCATTGACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGA31550.1180395041368076No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG31490.1178150233048517No Hit
GGATGGGGAAACCTTCCAGCTGATGGGGCTCTATGGCCGAGAACCAGATT31490.1178150233048517No Hit
GGGTATATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT30750.11504642637739568No Hit
GTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCATAG30210.11302609888979263No Hit
GTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTTAATGT29880.11179145431403521No Hit
GTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTCTCGGCCA28560.10685287601100554No Hit
CCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTTT28490.10659098170705701No Hit
CTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCAC28260.10573047185122608No Hit
ATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAACC27940.1045332407474613No Hit
GTCCTGACAGACAGACAATCCTATTCCCTACCAAAATGAAGATGCTGCTG27830.10412169255554218No Hit
GTAAATGTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTC27570.10314894228373328No Hit
TCACAGAATATTCACCAGCCTTTTCTGTTTTGTCAGCAACCATAGATAAT27450.10269998061982152No Hit
CTCCAGGCCCGAGAATGAAGAATGGCCTGAGCCTCCAGTGTTGAGTGGAG27290.10210136506793913No Hit
GGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTTGTGCA27130.10150274951605674No Hit
CATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTC26970.10090413396417436No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15000.064.6238861
GTGCAAG16850.046.3580631
GTATCAA68250.043.6433561
TCAACGC69100.041.68834
CAACGCA69350.041.5380175
AAGGGTA16200.041.481345
ATCAACG69500.041.3807533
AACGCAG72000.039.878656
TATCAAC72800.039.6370432
TGCAAGG17600.038.1845442
CTAGACG500.001703704637.5942763
GGTAATC17950.037.4631278
GTACATG109400.035.528741
AGGGTAA19350.034.971426
ACATGGG113950.033.9404223
ACGCAGA84800.033.8038147
CTTAGGT58500.033.0958143
GTACTAG2150.032.829921
CGCAGAG87400.032.7133188
CAAGGGT23000.032.6906744
Produced by FastQC (version 0.11.2)