FastQCFastQC Report
Sun 14 Apr 2019
SRR6575697_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575697_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2521024
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA235090.9325178974892743No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC130880.519154121499835No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC115550.45834549770252087No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC98330.39003992028635986No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC93740.371833032926303No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG77620.30789076184915337No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT72470.287462554898327No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA61280.24307582950420145No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA59340.23538054377903583No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC56930.22582093625447436No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT50010.19837177273996598No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT49690.19710244725952628No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT48030.19051782132974537No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG46960.18627351425452515No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT45060.17873689421441444No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC43750.17354059302886446No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA42730.16949461805996294No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA42370.16806662689446827No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA41200.16342565560661065No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG40100.1590623492675992No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT38670.15339005102688433No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT38550.15291405397171942No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA34730.13776148104897057No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG34700.13764248178517935No Hit
GTTTGGGGCTGACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACG34040.1350244979817725No Hit
GCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCA33190.13165285217435455No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA32950.1307008580640248No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA32460.1287572034221015No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA32350.12832087278820034No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC32270.12800354141809042No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT31730.12586155466984844No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC31270.12403689929171638No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30910.12260890812622173No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA30340.12034792211418852No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG29190.11578628366885836No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACATAGTT28520.11312863344418776No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT28410.11269230281028661No Hit
GGATGGAATGCAGATCACAGAATTTATACTGGGGATGCTGTATGGATAGG28320.11233530501891295No Hit
GGGTATATCAAAGAGAAATTTAAAGAAGTCTTTGATTCTTGATGAGGTAT28310.11229563859764921No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA28220.11193864080627555No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG28030.11118497880226448No Hit
GGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTC27590.1094396562666599No Hit
GGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAG27450.10888432636896753No Hit
GAATTATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGT26950.1069010053057805No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT26760.10614734330176945No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA26510.10515568277017592No Hit
ACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAG26350.10452102002995607No Hit
ATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCAT26050.10333102739204386No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTTCTCACA26030.1032516945495164No Hit
ATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCC25360.10059404432484578No Hit
GGTGAATATTCTGTGACGTATGATGGATTCAATACATTTACTATACCTAA25340.10051471148231829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16450.070.03141
GTATCAA76450.045.5757031
TCAACGC82700.040.8033834
ATCAACG82950.040.6804053
TATCAAC83800.040.568312
CAACGCA85250.039.748265
AACGCAG87450.038.7483026
GTGCAAG20350.036.969891
AAGGGTA16800.036.926825
TTAGGTA39650.033.307374
GGTAATC18400.033.2049458
TAGGTAT39600.033.1120645
CTTAGGT39500.033.076913
AGGTATA40250.032.6941036
GTACATG129350.032.6075861
GTCTTAG42450.032.5658381
ACGCAGA106800.031.7278947
TACATGG133850.031.6167182
CGCAGAG106900.031.6102878
ACATGGG134550.031.3667743
Produced by FastQC (version 0.11.2)