FastQCFastQC Report
Sun 14 Apr 2019
SRR6575703_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575703_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654091
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9970.1524252741591002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAA802.456527E-741.1247374
CGCTAAA802.456527E-741.1247375
GTATCAA14000.037.2839971
CCCGCTA906.185146E-736.5553173
GTACATG31550.034.132151
ACATGGG30600.034.097823
TACATGG32150.033.333592
CATGGGG17350.030.6107524
CAACGCA17100.029.9588685
AACGCAG17500.029.8112356
TCAACGC17350.029.7980794
ATCAACG17200.029.7846893
GGTTAAC650.00613638128.9383741
GATACTA700.00881053926.8713471
GTACGAG700.00881053926.8713471
ATGGGAG6550.025.8318925
GTACATA1659.257383E-825.6499231
GGGTACC5000.025.3798357
ATGGGTA5050.025.128555
CTAAGAC3600.024.8053953
Produced by FastQC (version 0.11.2)