FastQCFastQC Report
Sun 14 Apr 2019
SRR6575703_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575703_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences654091
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10970.16771366675279128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14700.036.218131
GTACTAG702.1526832E-433.6540151
GTACATG28800.030.9196241
TACATGG29000.030.4738482
ACATGGG29550.029.424923
TCAACGC17850.028.9637974
GTATCGC650.006154333228.9210769
CAACGCA18400.028.3534685
GTACTAT1005.2317177E-528.2693711
ATCAACG18300.028.2515743
CATGGGG17400.028.092164
AACGCAG18750.027.8242036
TAATACC1406.3608604E-726.857344
GTACATA1501.1366428E-625.128331
GGTATCG950.001304510324.735138
TCTAGGG1151.3762535E-424.5219173
TATCAAC21700.024.0453282
AGTATAG1000.001757797323.5001725
GGGTACC4800.023.4983777
GGTACCT4850.023.2561248
Produced by FastQC (version 0.11.2)