FastQCFastQC Report
Sun 14 Apr 2019
SRR6575704_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575704_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601913
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11600.19271888130012146No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7250.1204493008125759No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6760.11230858944731215No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6350.10549697381515269No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6260.10400174111541037No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6190.10283878234894411No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12400.050.431791
ATCAACG17750.034.422233
GTACATG32600.033.8941461
CAACGCA18400.032.9507945
ACATGGG33850.032.7678033
TACATGG34000.032.63682
TCAACGC18750.032.5863764
AACGCAG18750.032.3357126
GGTATCA5300.031.937511
TATCAAC22800.027.6340392
CTTACTA1205.8375226E-627.4164249
CATGGGT8500.027.093884
ACGCAGA22650.026.7679757
GATATAG1257.990342E-626.3307061
ATATAGG1859.973519E-925.4157372
CATGGGG18050.025.2573934
CGCAGAG24200.025.0534978
CATGGGC5800.024.310134
ATGGGAG6800.024.1909625
ATACCGT1751.6436024E-724.1712156
Produced by FastQC (version 0.11.2)