FastQCFastQC Report
Sun 14 Apr 2019
SRR6575704_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575704_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences601913
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10870.18059088273554486No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6790.11280700034722625No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6710.11147790461412198No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6450.10715834348153305No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11850.054.8953481
GGTATCA4850.046.6524281
GTACATG33550.039.2000351
TACATGG34250.038.1468122
ACATGGG33300.038.106023
ATCAACG17050.038.038973
TCAACGC18000.036.814654
CAACGCA18200.036.1518675
AACGCAG18950.034.225046
CATGGGA14750.032.818544
ATGGGAG8750.031.689695
TATCAAC20350.031.4085962
ACGCAGA21400.030.306757
GTATAGG1751.6552804E-1029.6298471
GTATAGA1005.2138286E-528.2830371
CGCAGAG23450.027.4569198
ATGGGTA5500.026.4894585
GTACTAG909.3505083E-426.1879961
GCAGAGT25450.025.6685339
CATGGGG17950.025.6587624
Produced by FastQC (version 0.11.2)