FastQCFastQC Report
Sun 14 Apr 2019
SRR6575713_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575713_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3329734
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA250620.7526727360203548No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC154670.464511579603656No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC136660.4104231749443048No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC103600.3111359646145908No Hit
CTATTATCCTGGCCTCTGACAAGAGAGAAAAGATAGAAGATAATGGCAAC90090.27056215301282327No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGTGTTTGGGGCT74490.22371156374653348No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG69280.2080646682287534No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA61900.18590073561431633No Hit
ACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT55550.16683014318861505No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG52810.1586012576379975No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT51820.15562804716532913No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC51350.15421652300153704No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT51280.15400629599841908No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA50770.15247464211855963No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG49710.14929120464277326No Hit
ACTTAAGACAGACTATGATAATTATATTATGATTCATCTCATTAACAAAA49460.14854039391735194No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC49230.14784964804996434No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA47560.14283423240415No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA45050.13529609272092005No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG44270.13295356325760554No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT43600.13094139051347647No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT42740.12835860161802715No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT39530.11871819190361753No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG38220.11478394370240987No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG37720.11328232225156723No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA36980.11105992250432016No Hit
GGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCATTCTC36660.11009888477578088No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35920.10787648502853381No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT35630.10700554458704509No Hit
GTTTGGGGCTGACCCTAGTGTGTGTCCATGCAGAAGAAGCTAGTTCTACG35550.10676528515491028No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA35360.10619466900359008No Hit
GTCCTGGTGAGAAGTCTCCACTCAACACTGGAGGCTCAGGCCATTCTTCA35050.10526366370406766No Hit
GATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTCAACAC35040.10523363127505081No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA34870.1047230799817643No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT34690.10418249625946097No Hit
TACTATACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTA34310.10304126395682058No Hit
CAAGAAAGACGTGGTCCTGACAGACAGACAATCCTATTCCCTACCAAAAT33720.1012693506448263No Hit
CTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGAAA33510.10063866963547238No Hit
GACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAA33330.10009808591316903No Hit
GACCTATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGC33300.10000798862611847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18550.055.1324961
GTATCAA81050.052.2755431
ATCAACG91900.045.2105643
TCAACGC92150.045.0852054
TATCAAC93450.044.36012
CAACGCA95100.043.78555
AACGCAG96700.043.206186
AAGGGTA15750.038.49345
ACGCAGA116950.035.4029737
CGCAGAG118400.035.0096248
GTACATG146400.034.960741
AGGGTAA18000.033.9423146
GGTAATC18300.033.1290668
ACATGGG155400.032.9064523
TACATGG154400.032.876052
GCAGAGT127550.032.6086969
GTGCAAG25750.031.4806041
GTCTTAG49550.031.197661
CTTAGGT44900.031.1941473
ATGGGGG49450.031.0783945
Produced by FastQC (version 0.11.2)