FastQCFastQC Report
Sun 14 Apr 2019
SRR6575715_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575715_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550767
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11770.21370198287115968No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7650.13889721061719384No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6630.12037758253490133No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6120.11111776849375507No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5900.10712333890737825No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5880.10676020894498037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12450.050.594461
CTTACTA906.196715E-736.5448239
ATCAACG17100.035.9985473
CAACGCA17850.034.7492525
AACGCAG17850.034.7492526
GTACATG30650.034.661351
TCAACGC17800.034.582874
TACATGG32350.033.130512
ACATGGG32200.032.9808853
CATGGGG17000.030.4055984
TATCAAC20800.029.8317072
ACGCAGA20800.029.8208737
TACAGCG650.006157522628.917217
CGCAGAG22550.027.7149988
GTATAAT2500.026.324221
TATAGGC1807.496055E-926.1058143
TCTATAC909.522632E-426.1058143
TAATACC1301.0881977E-525.3025594
GCAGAGT25350.025.0222389
GATATAG1351.4538415E-524.374281
Produced by FastQC (version 0.11.2)