FastQCFastQC Report
Sun 14 Apr 2019
SRR6575715_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575715_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences550767
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11270.20462373381121235No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.12727705182046128No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6890.12509827204607393No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6630.12037758253490133No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5670.1029473443398025No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5560.10095012954661409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11850.052.8744541
ATCAACG16150.037.8283583
GTACATG31450.036.998911
CAACGCA16800.036.0850335
TCAACGC16900.035.871514
ACATGGG31900.035.06173
TACATGG33450.034.560952
AACGCAG17800.033.793776
GTTATAC702.1537647E-433.6499631
ATAGTGC753.2756064E-431.3296416
CTAGTAC600.004161516731.329643
TATCAAC20250.030.1692872
TAGTACT1252.3618486E-730.0764544
TTAGGCA650.006154965628.9196684
CCTATAC650.006154965628.9196683
ACGCAGA20500.028.8844017
ATGGGTA5050.028.8480855
CATGGGG16200.028.428754
CATGGGA11950.028.3146534
GGGTACC4350.028.0886447
Produced by FastQC (version 0.11.2)