FastQCFastQC Report
Sun 14 Apr 2019
SRR6575720_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575720_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3140948
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA207710.6612971625127191No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC170770.5436893574806078No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC137130.4365879345980895No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC93020.29615262653186236No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG71140.22649212912789385No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG64130.204174026440425No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT61550.19595994585074314No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT57080.18172857366629439No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT55190.17571128207152745No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA55090.1753929068548731No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC54230.17265487999164583No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG50600.1610978596270935No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA49540.1577230823305575No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT49380.15721368198391059No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA48100.15313847921073512No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA47840.15231070364743385No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT46830.14909511395922503No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT46210.14712118761596818No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC45780.14575217418435454No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT44120.14046714558789256No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA43520.13855689428796655No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT40370.128528074963355No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA38880.12378428423520543No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG36260.11544285355886184No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT35940.11442405286556798No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG32640.10391767071597494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17600.060.8962971
GTATCAA74400.054.2104571
TCAACGC85150.046.08874
ATCAACG85950.045.7144053
CAACGCA88800.044.2472155
TATCAAC89300.044.1124572
AACGCAG92200.042.7168126
GTACATG154650.034.621231
TACATGG157700.034.0708732
ACGCAGA115500.033.8960237
ACATGGG163850.032.843673
TAGGTAT44400.031.8622285
AGGTATA44900.031.7162636
CTTAGGT45000.031.6462863
CGCAGAG124450.031.382818
TTAGGTA45450.031.3329564
GTCTTAG48650.031.3062381
AGGGTAA16150.030.8474356
TCTTAGG51350.030.026322
GCAGAGT130700.029.9899839
Produced by FastQC (version 0.11.2)