FastQCFastQC Report
Sun 14 Apr 2019
SRR6575721_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575721_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3033373
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA213970.7053863801121721No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC165340.5450697952411392No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC139260.45909289757639427No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC93510.3082706940425724No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG70960.2339310068362842No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG64820.21368951329098004No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT61660.20327206710154008No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC57420.189294227910646No Hit
GTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGACAGACAATCCT56470.18616240073344095No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA54600.17999764618462682No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT53320.17577792114586635No Hit
GGATAATAGTATGCCATTCCCCATTAATCTTTTCTACATTAAAGTTCCTT52300.1724153277556041No Hit
GAATTGGACTTCTCAGTGAGACAGGATGGAATGCAGATCACAGAATTTAT49180.1621297479736254No Hit
GTACATGGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAG49010.16156931574191502No Hit
GCATTGGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACA48550.16005285205611047No Hit
CTGTAAGAGATGAAGAGTGCTCCGAATTATCTATGGTTGCTGACAAAACA48420.15962428623186137No Hit
GGCCTGGAGGCAGCGATTGGCATTGGATAGGTCAATGATATTTTCTCTAA46720.15401996391475758No Hit
ATCTATGGTTGCTGACAAAACAGAAAAGGCTGGTGAATATTCTGTGACGT46400.15296503265506747No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT45840.15111890295060976No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC43760.144261849762624No Hit
GTATTGAATCCATCATACGTCACAGAATATTCACCAGCCTTTTCTGTTTT42120.1388553270567121No Hit
GTACATGGGGAGTGTAGCCACGATCACAAGAAAGACGTGGTCCTGACAGA40060.13206420707245695No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA39380.12982247814561546No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGCTGCTGCTGCTGTGTTTGGG37270.12286652515203372No Hit
ACACTGGAGGCTCAGGCCATTCTTCATTCTCGGGCCTGGAGGCAGCGATT36320.11973469797482868No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA33270.10967988440590722No Hit
GACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAAAGGATGGGGA33060.10898758576673556No Hit
ATTCAATACATTTACTATACCTAAGACAGACTATGATAACTTTCTTATGG33020.10885571935927432No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG31660.10437226150559131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19250.057.1326331
GTATCAA70300.050.6772461
TCAACGC79400.043.4363174
ATCAACG79350.043.4044723
CAACGCA82800.041.6527025
TATCAAC84100.041.1879962
AACGCAG84750.040.8052066
GTACATG143450.036.8596421
TACATGG146300.035.8524632
ACATGGG152950.034.4069333
ACGCAGA104500.033.0482527
CATGGGG107400.031.6746434
CGCAGAG113900.030.3620828
GTCTTAG41850.030.2103631
AGGGTAA16350.030.1751176
CTTAGGT39950.029.8740713
ACCTAAG79450.029.6375961
TAGGTAT40100.029.5279675
GTACTTG19050.029.3596691
AAGGGTA17000.029.2977585
Produced by FastQC (version 0.11.2)