FastQCFastQC Report
Sun 14 Apr 2019
SRR6575722_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575722_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181404
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11840.10021973854837127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15350.049.391141
ATCAACG20050.036.336183
TCAACGC20450.036.0836034
CAACGCA20850.034.937555
GGTATCA6800.033.240221
AACGCAG22150.033.0992136
GTACATG43550.031.7900141
TACATGG44700.030.9144632
ACATGGG44900.030.043983
AGGTATA3000.029.7645366
GTCTTAG5200.028.0730691
TATCAAC27000.028.0274772
GTACCGT1205.8466176E-627.4147016
TTAGGTA3400.026.2650494
TAGGTAT3250.026.0289065
ACCTAAG5650.025.8371621
ACGCAGA28800.025.7817847
TACCGTG1301.0888225E-525.3048117
CGCAGAG31000.024.1037068
CATGGGG29250.024.1028794
Produced by FastQC (version 0.11.2)