FastQCFastQC Report
Sun 14 Apr 2019
SRR6575724_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575724_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034020
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA14220.13752151795903367No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12910.1248525173594321No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12780.12359528829229609No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11860.11469797489410262No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11110.10744473027601013No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG10670.10318949343339587No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12400.052.3184051
TCAACGC17350.036.0265964
ATCAACG17800.034.851783
CAACGCA18300.034.1563645
GTACATG37900.032.4981881
TACATGG37650.032.2145272
AACGCAG20150.031.020426
ACATGGG40050.029.8059023
TATCGAT404.771743E-429.38597380-81
GGTATCA5150.027.3848951
GTCTTAG4700.026.005941
CATGGGG24850.025.5316244
CTAAGAC5600.025.1770173
TATCAAC25350.025.0353032
ACGCAGA25900.024.1336447
ACCTAAG4350.023.7755471
ATATTAG1000.001756224323.505371
ACTACGC1602.0395692E-623.4985493
TAAGACA6600.023.4985494
CGCAGAG27450.022.9421188
Produced by FastQC (version 0.11.2)