FastQCFastQC Report
Sun 14 Apr 2019
SRR6575724_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575724_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1034020
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13550.13104195276687106No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13150.1271735556372217No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12980.12552948685712076No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12100.11701901317189223No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG11180.10812169977369877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13850.055.8287161
TCAACGC17200.043.9961554
ATCAACG17750.043.1666683
CAACGCA17950.042.1558385
AACGCAG18850.040.392436
GTACATG37900.034.5835151
TACATGG38050.033.726192
ACATGGG39800.032.3613623
ACGCAGA24100.031.5917177
TATCAAC25150.030.6524492
ACCTAAG3650.029.7097191
CGCAGAG25850.029.4530168
CTAAGAC6100.029.2828313
TAAGACA6900.027.9287874
CATGGGG25700.027.0673584
CTATTAC1406.1947685E-726.9417321
CGATATA350.00883986526.85574566-67
GTCTTAG4750.026.7999341
GCAGAGT30200.025.366239
GGTATCA5450.024.2228411
Produced by FastQC (version 0.11.2)