FastQCFastQC Report
Sun 14 Apr 2019
SRR6575725_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575725_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930389
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13100.1408013207378849No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11320.12166953822540894No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11110.11941241781663368No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10920.11737026125631321No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10470.11253357466608054No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA9570.10286020148561517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12900.046.6368061
TCAACGC17050.035.2739454
ATCAACG17150.034.7942963
CAACGCA17400.034.294385
AACGCAG18450.032.5973326
CTTAGGT2450.030.6847343
TAATACC2000.030.5409014
GTACTAG804.779502E-429.3757211
TACATGG33950.029.2113232
GTACATG34800.029.038071
ACATGGG34600.028.5175153
TAGGTAT2053.6379788E-1127.5045
GTCTTAG3850.026.8578051
AGTGCGT700.00884801826.849146
TTAGGTA2850.026.3781074
ACGCAGA23250.026.0696537
TATCAAC23400.025.9108942
AGGTATA2301.546141E-1024.5144356
GGTATAG2352.0190782E-1023.9928537
ATAATAC2402.6375346E-1023.4933
Produced by FastQC (version 0.11.2)