FastQCFastQC Report
Sun 14 Apr 2019
SRR6575726_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575726_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences362347
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9450.2607997306449343No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6820.18821737174586792No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5690.15703179548885463No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5670.15647983838696056No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5570.15372005287749038No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5510.15206418157180823No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4680.12915796184320555No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4630.12777806908847045No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4450.12281045517142407No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4390.12115458386574196No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4160.11480707719396048No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG4100.11315120588827837No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC4090.11287522733733134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4090.11287522733733134No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3930.10845957052217901No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3860.10652772066554989No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC3670.10128412819755649No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3660.10100814964660947No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3650.10073217109566246No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGT250.00522850256.4126744
GTATCAA9550.048.8886831
CATAACA601.5995938E-647.0105632
TAGGTAT1051.1314114E-940.294765
AACGCAG12300.038.602176
TTAGGTA1101.7862476E-938.463194
CAACGCA12100.038.4631845
ATCAACG12000.038.3919563
TCAACGC12050.037.8425254
CATGGGG10250.036.2325334
TACATGG20850.035.173372
GTACATG21050.034.9501461
TATACCA550.002711574534.18955
CTTAGGT1256.2282197E-933.8476073
ACATGGG21500.033.4540253
ACGCAGA14700.031.9711487
CGCAGAG14900.031.5420048
GTCGGTC600.004158301331.3317248
TATCAAC15650.030.0386962
TAACGCC1452.6437192E-829.1789684
Produced by FastQC (version 0.11.2)