FastQCFastQC Report
Sun 14 Apr 2019
SRR6575727_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575727_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682307
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11310.16576116029880977No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10290.1508118779376439No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10010.1467081533679121No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9660.14157849765574734No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9060.13278480214917918No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA8530.12501703778504397No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG8340.1222323675412974No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT7400.10845557791434061No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7380.10816245473078835No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA7070.10361904538572812No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6970.10215342946796678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10850.058.0482561
GTACTAG250.00523435456.402111
TCAACGC13850.044.7926184
CAACGCA14100.043.998425
ATCAACG14450.042.9327133
AACGCAG14950.041.4968386
GGTATCA3300.039.880281
TAGGTAT1700.035.9398885
ACGCAGA18050.034.3699577
TATAGTA1800.033.9432269
AGGTATA1850.033.025846
TATCAAC19250.032.7181052
TTAGGTA1900.032.1567424
CTTAGGT1900.032.1567423
GTACATG26500.031.925721
TACATGG26850.031.3345072
GTCTTAG2850.031.3345051
GGTATAG1950.031.3322097
ACATGGG27350.030.587573
CGCAGAG20550.030.4174028
Produced by FastQC (version 0.11.2)