FastQCFastQC Report
Sun 14 Apr 2019
SRR6575728_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575728_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646618
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10860.1679507839249758No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA10150.1569705761361422No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9350.14459851102196353No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG8670.1340822556749116No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8490.13129854102422142No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG8140.12588576253676823No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7930.12263809544429632No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7670.11861717428218825No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7580.11722531695684314No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7400.11444160230615294No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT7180.1110392843997538No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6640.10268814044768317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8400.057.6454661
ATCAACG12400.039.0320363
TCAACGC12400.038.6530844
CAACGCA12900.037.152035
AGGTATA1302.3101165E-1036.1433756
AACGCAG13400.036.1164056
GGTATCA2900.035.664151
GTACATG22900.032.2308241
TTAGGTA2200.032.0386544
GTGTTAA600.00415452431.3412251
GGTATAC600.00415452431.3412251
CTTAGGT1658.731149E-1131.3266833
TACATGG23450.031.27442
GTCTTAG2750.030.7713831
ACGCAGA15900.030.4377257
ACATGGG23750.029.4800553
TAGGTAT1602.1373125E-929.3664935
TACTAAA650.006144638628.9303632
TATCAAC17250.028.61592
CGCAGAG17250.028.0556418
Produced by FastQC (version 0.11.2)