FastQCFastQC Report
Sun 14 Apr 2019
SRR6575728_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575728_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences646618
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9530.14738222567265372No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9510.14707292404479924No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG9400.1453717650915997No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9150.14150549474341884No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8410.13006133451280355No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG7870.12171019056073293No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7500.11598811044542527No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG7360.11382299905044402No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7340.11351369742258954No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA7240.1119671892833172No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7240.1119671892833172No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT6950.10748231567942741No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6930.10717301405157297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9350.051.3402751
GTATTAT1003.284731E-837.6495321
TCAACGC13700.034.9873164
GGTATCA4100.034.4355471
CAACGCA14400.033.6128855
ATCAACG14500.033.0569843
AACGCAG15200.031.8437846
TACATGG26300.030.7376672
GTACATG27200.030.2788051
ACATGGG27650.029.4023483
ACGCAGA17600.027.5014517
TATCAAC18050.026.8199672
CTTACAT1101.018726E-425.6324213
CATGGGG16750.025.5304014
CGCAGAG19350.025.031668
GGTACCT3250.023.1510148
ATGGGAG5900.023.0981435
ATTATCC2853.6379788E-1223.084173
GCAGAGT21250.022.7935359
AGAGTAC18250.022.67530810-11
Produced by FastQC (version 0.11.2)