FastQCFastQC Report
Sun 14 Apr 2019
SRR6575729_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575729_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128811
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18750.056.150421
TCAACGC26400.038.987664
ATCAACG26650.038.4455683
CAACGCA27550.037.1896365
AACGCAG28250.036.2681246
TATCAAC31800.032.664152
GTACATG49500.030.8591821
ACGCAGA33500.030.584317
TACATGG50200.029.867112
ACATGGG50700.029.8493543
GCGTAGT1154.195672E-628.6092471
CGCAGAG36800.027.969418
GGTATCA7300.025.1100731
GCAGAGT41900.024.6771879
AGAGTAC34800.024.64737310-11
ACCTAAG1751.6458216E-724.1718961
CATGGGG27600.023.8399874
CATGGGA27850.023.2884654
GTAATAC1852.8153408E-722.8642923
CATATAG2005.9643025E-721.1494733
Produced by FastQC (version 0.11.2)