FastQCFastQC Report
Sun 14 Apr 2019
SRR6575731_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575731_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705466
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43480.6163301987622366No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT36650.5195147604562091No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG15390.21815367430889654No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG13070.18526761034550213No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11970.16967508001803064No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10680.15138929445217772No Hit
ACCTATACCCGGCCGTCGGGGCAAGTTCTAGGCCCCGATGAGTAGGAGGG9980.14146677515287767No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATG9780.13863176963879195No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC9580.13579676412470623No Hit
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGG9400.1332452591620291No Hit
GTACATGGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCA9300.1318277564049862No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC9170.12998500282083048No Hit
GTCTGATAGCGTGCTAAGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATT8920.1264412459282233No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCA8400.11907023159160043No Hit
AGGTAGGACGGCGCGGCTGCTTCGTTGAGCCGCGCCAAGGAATCGAGAGC8150.11552647469899328No Hit
CCCTAACGTTGCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCG8130.11524297414758472No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC8000.11340022056342901No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTT7440.10546220512398896No Hit
CTATCAGACGGGCTTCCCCCGTCTCTTAGGATCGACTAACCCATGTGCAA7200.10206019850708609No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCCCT7170.10163494767997325No Hit
GTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCCCA7140.10120969685286038No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTGGGGCGCGAGCCCGGGC7140.10120969685286038No Hit
GAGTACGACCGGGCGTGGGAGGCACTCGGTCCTCCGGATTTTCAAGGGCC7070.10021744492293037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAC5050.032.5723658
CATGGGG24050.030.4847184
GTTATAC953.7517304E-529.6824873
TATATAC1005.322763E-528.1983643
ATAAGGT3400.026.263183
TGATCGC3450.025.8825559
GTATAAG4200.025.75121
TGGGGGG5550.025.403936
ATGGGGC7750.024.8630735
TTATACC1151.3770143E-424.5203154
GGGCTAT2701.8189894E-1224.3689586
GGCTATT2751.8189894E-1223.9258827
ATGGGGG13400.023.8493635
GGACCCC5450.023.28305294
CTATTGA3050.023.1134139
GTGATCG3900.022.8961078
TAAGGTG3900.022.8961074
GTAATAC1652.6627586E-622.7865563
GTATTAT1050.002327713222.3923471
ATGGGCG2154.9418304E-821.8591945
Produced by FastQC (version 0.11.2)