FastQCFastQC Report
Sun 14 Apr 2019
SRR6575731_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575731_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences705466
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA41030.5816013812146864No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT36970.5240507692787462No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG15520.21999642789305224No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG12240.17350233746204635No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC11620.16471382036838061No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10640.15082229334936056No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10540.1494047905923177No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATG10470.1484125386623877No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC9050.12828399951237904No Hit
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGG8800.12474024261977189No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC8770.12431499179265905No Hit
GTACATGGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCA8600.12190523710568617No Hit
GTCTGATAGCGTGCTAAGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATT8340.11821972993737472No Hit
ACCTATACCCGGCCGTCGGGGCAAGTTCTAGGCCCCGATGAGTAGGAGGG8310.11779447911026188No Hit
CCCTAACGTTGCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCG7430.10532045484828469No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTT7390.10475345374546753No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCA7310.10361945153983323No Hit
AGGTAGGACGGCGCGGCTGCTTCGTTGAGCCGCGCCAAGGAATCGAGAGC7280.10319420071272038No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA1303.2421121E-729.0101171
CTAGTAT700.00883579626.8558678
CATGGGG22700.026.0905634
TATACCG1101.01713864E-425.6387795
AGTACTT13150.024.3030312-13
ATGGGGC8200.023.5022145
ATGGGAC3600.023.5022145
TAGGCAT3050.023.1169345
GGGCGAC4750.022.7568158
ACGCAAG1050.002335253422.3798898
TACTTTT14300.021.69127714-15
GGGCTAT2701.1386874E-920.8908586
GTAGGAC10000.020.681953
ATGGGCG2351.262506E-720.0018865
GTACTAG3353.8198777E-1119.70091
CTTTTTT15750.019.69430416-17
ATACCCG7400.019.6910445
GTACTGG2401.5285332E-719.642271
GTATATA1200.00497501419.642271
ACTTACT600.00506608319.58240338-39
Produced by FastQC (version 0.11.2)