FastQCFastQC Report
Sun 14 Apr 2019
SRR6575732_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575732_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652579
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA48380.7413661794204227No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT39400.6037583189161773No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG16680.25560123755131564No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG14140.21667874694098338No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG12330.18894264142732145No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC12090.1852649257791011No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC11700.17928863785074298No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATG10540.16151301221767786No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC9690.1484877692968974No Hit
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGG9040.13852728941630055No Hit
GTACATGGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCA9010.13806757496027303No Hit
ACCTATACCCGGCCGTCGGGGCAAGTTCTAGGCCCCGATGAGTAGGAGGG8690.1331639540959792No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCA8520.1305589055118231No Hit
GTCTGATAGCGTGCTAAGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATT8500.13025242920780472No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC7960.1219775689993089No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCCCT7130.10925880238254679No Hit
GAGTACGACCGGGCGTGGGAGGCACTCGGTCCTCCGGATTTTCAAGGGCC7120.1091055642305376No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGA7080.10849261162250087No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTT6970.10680699195039989No Hit
AGGTAGGACGGCGCGGCTGCTTCGTTGAGCCGCGCCAAGGAATCGAGAGC6930.10619403934236316No Hit
CTATCAGACGGGCTTCCCCCGTCTCTTAGGATCGACTAACCCATGTGCAA6920.10604080119035397No Hit
CCCTAACGTTGCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCG6770.10374222891021624No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAGTC550.002719899934.1722074
ATGGGTC1900.032.148855
TCTAACT1351.3289537E-831.3245223
CATGGGG21700.029.4479374
TATATCT1853.2923708E-1027.938094
ATGGGGC6600.025.6291545
TATAGAC1101.0196229E-425.6291543
GGGCTAT3650.024.4588746
TGGGGGA6200.024.2512446
CTTAGGG1401.9338617E-523.4933913
CTAGGTA1000.001760747523.493394
ATGGGCG3650.023.1715665
ACTGTGC3300.022.7814718
CTATTGA3750.022.5536569
GGCTATT3550.022.5007137
ATTCTGG3350.022.4414488
TCCTACC3150.022.3900992
GTAGTAA1050.002328886422.3900991
TAGTACT2957.2759576E-1222.2988134
AGGGCTA3800.022.2568955
Produced by FastQC (version 0.11.2)