FastQCFastQC Report
Sun 14 Apr 2019
SRR6575732_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575732_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences652579
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA45410.6958544482736956No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT41630.6379304268142247No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG16580.2540688560312238No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG13870.2125413168367355No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC12490.19139445185946835No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10940.16764253829804515No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10860.1664166330819717No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC9770.14971367451297085No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATG9490.14542300625671373No Hit
GTACATGGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCA8710.13347043039999756No Hit
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGG8420.12902652399173126No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC8190.12550204649552008No Hit
ACCTATACCCGGCCGTCGGGGCAAGTTCTAGGCCCCGATGAGTAGGAGGG7800.11952575856716198No Hit
GTCTGATAGCGTGCTAAGCGCGAGCTTCGAAAGGGAATCGGGTTAAAATT7650.11722718628702426No Hit
TCGCAATGCTTTGTTTTAATTAAACAGTCGGATTCCCCTTGTCCGTACCA7200.11033146944661107No Hit
AGGTAGGACGGCGCGGCTGCTTCGTTGAGCCGCGCCAAGGAATCGAGAGC7150.10956527868656515No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC6840.10481489597428051No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTT6800.10420194336624378No Hit
CCCTAACGTTGCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCG6720.10297603815017033No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC550.002717975834.1771353
CATGGGG20950.029.8336224
GGCCTAT804.7163226E-429.4409261
GTCTAGC1005.2393796E-528.2632881
GTATAAG3250.027.5385881
TACTTGG3050.026.1931322
GAGTGCG909.478343E-426.1275589
GTGATCG2900.025.947378
ATAAGGT2950.025.4880333
TAAGGTG3150.025.3616054
GTACTTG5800.025.1770671
ATGGGCG3050.024.652365
ATGGGGT4400.024.5648175
AGGTCGC4800.024.4758136
GTCCTAC3400.023.552741
TTAGGTA1201.8385661E-423.496784
ATGGGGC5850.023.2959545
GGTCGCG5050.023.2641377
TGATCGC3450.023.174019
ATGGGGG12400.022.7388215
Produced by FastQC (version 0.11.2)