FastQCFastQC Report
Sun 14 Apr 2019
SRR6575733_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575733_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718814
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA10030.13953540136947803No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9410.1309100824413548No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT9360.13021449220521583No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9230.12840595759125448No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8560.11908504842699223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9850.041.51771
CTAAGAC4400.034.1811833
TAAGACA4950.031.3327544
ACCTAAG3850.030.5231631
ATCAACG13650.028.5782263
TCAACGC13750.028.3703844
GTACATG28500.027.7086051
CAACGCA14100.027.6661555
GTCTTAG3450.027.2496641
TACATGG28800.027.0935442
GGTATCA6100.026.9704671
ATACGCT3850.026.8566468
TATACGC3850.026.8566467
AACGCAG14850.026.2688756
TACGCTA3950.026.1767319
TAGATAG1101.0179446E-425.635895
CCTAAGA4800.025.4614032
CGCGCAT751.1630582E-625.06620462-63
ACATGGG30600.025.0354843
GTATTAC950.001303193924.7398241
Produced by FastQC (version 0.11.2)