FastQCFastQC Report
Sun 14 Apr 2019
SRR6575733_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575733_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718814
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA9920.1380051028499723No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7970.11087708364055236No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7630.10614707003480733No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7470.10392118127916262No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7350.1022517647124291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10600.052.8716741
ATCAACG13750.040.3323973
TCAACGC13800.040.1862644
GGTATCA3850.039.1445431
CAACGCA14300.038.781155
AACGCAG15650.035.435816
CTTAGGT3000.032.8982473
GTATACA1658.367351E-1131.3971861
TATCAAC18600.029.3102662
AGGTATA2900.029.1708586
TAGGTAT2900.029.1708585
ACGCAGA19150.028.9592937
TTAGGTA3100.028.8049164
GTACATG29450.028.4653591
TACATGG29000.028.3605582
ACATGGG30650.026.8338073
CGCAGAG20500.026.822968
CCTTATA1659.312316E-825.6349982
CATGGGG18100.024.6671944
GGTATAG3450.024.5204327
Produced by FastQC (version 0.11.2)