FastQCFastQC Report
Sun 14 Apr 2019
SRR6575734_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575734_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667643
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA9820.14708459461119192No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9180.13749863325160303No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8340.12491705896714263No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA8320.12461749767465546No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8200.12282012991973255No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC6740.10095215556817042No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11650.045.9819641
ATTTACG250.001704472337.58945526-27
ATCAACG14350.035.36293
TCAACGC14350.035.36294
GGTATCA5350.033.3763161
CAACGCA15300.033.1671685
AACGCAG15500.033.0423436
GTACATG29100.031.1654131
TACATGG29400.030.5277372
GTATAGG1102.9707426E-629.9029431
ACATGGG29900.029.8578453
TATCAAC18550.028.1182082
ACGCAGA18550.027.6095057
GTATACA1057.427379E-526.8516221
CTAAGAC5650.026.6120033
AGGTATA2305.456968E-1226.5577686
TAAGACA5950.026.0599164
TAGGTAT2601.8189894E-1225.3005945
TATTAAG1501.1633929E-625.0615142
CGCAGAG20300.024.9979138
Produced by FastQC (version 0.11.2)