FastQCFastQC Report
Sun 14 Apr 2019
SRR6575734_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575734_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667643
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA9970.14933130430484554No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7900.11832671053242526No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7410.11098745886649003No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7400.11083767822024645No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7240.10844118788034922No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC6810.10200062009187545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8600.050.8644561
GGTATCA3700.038.1372531
ATCAACG11750.036.39513
TCAACGC11950.035.7859764
CAACGCA12550.034.0750925
AACGCAG13050.033.129646
TACATGG25150.030.0856762
GTACATG25950.029.1822221
AGGTATA2101.8189894E-1229.091326
ACATGGG26350.029.0700873
TAGGTAT2300.028.6048435
ACGCAGA16000.027.3150737
ACCTAAG4550.026.8776841
TATCAAC17000.025.9866832
GTATTAC1301.0805108E-525.3270451
CGCAGAG17250.025.0801988
TATAATG2751.8189894E-1223.9258422
TTAGGTA2751.8189894E-1223.9240514
TAAGACA6100.023.8820294
GTCTTAG3950.023.815671
Produced by FastQC (version 0.11.2)