FastQCFastQC Report
Sun 14 Apr 2019
SRR6575735_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575735_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1141117
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA27840.2439714770702741No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC23980.2101449719879732No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC19080.16720458988867926No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14500.12706847764076779No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13890.12172283823656996No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13570.1189185683851875No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12350.10822728957679187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19050.053.303441
TCAACGC24450.040.7510954
ATCAACG24600.040.502613
CAACGCA25350.039.304315
GGTATCA7200.039.175561
AACGCAG26000.038.32176
TATCAAC29750.033.6580622
GTACATG48650.031.1149771
ACGCAGA32350.030.7995137
TACATGG49600.030.2346882
ACATGGG50900.029.4547373
GTCTTAG7150.028.9296441
TACGCAT650.006155228728.9220395
CGCAGAG34600.028.7966568
TAGGTAT6950.027.7256245
TTAGGTA7300.027.0401254
ACCTAAG11300.026.6255151
AGGTATA7300.026.3963136
GGTATAG7650.025.8029967
CTAAGAC12800.025.7022043
Produced by FastQC (version 0.11.2)