FastQCFastQC Report
Sun 14 Apr 2019
SRR6575736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031802
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA26970.2613873592026377No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21430.2076948871973499No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC19530.18928050149156525No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13700.13277741272065766No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13140.1273500148284264No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12300.11920891799007949No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11100.10757877964958393No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10490.10166679265983201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19000.047.7539251
TCAACGC23150.038.5634964
ATCAACG23400.037.9506953
CAACGCA24100.036.8483925
AACGCAG24950.035.7813576
GTACATG47300.031.9043051
TAGGTAT6750.031.3243875
TACATGG48150.031.1458192
TATCAAC29400.030.7014772
ACATGGG48850.030.3945923
GGTATCA7600.030.3101311
AGGTATA7000.030.2056586
TTAGGTA7050.029.9914324
GTCTTAG7850.029.3448431
ACGCAGA31350.028.4767137
CATGGGG29100.028.0950684
CTTAGGT7700.028.0699023
CGCAGAG32450.027.3666048
GGTATAG7900.027.3592747
ACCGTCC1555.0964445E-827.2825288
Produced by FastQC (version 0.11.2)