FastQCFastQC Report
Sun 14 Apr 2019
SRR6575737_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575737_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328121
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9690.29531788577994095No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6300.1920023405999616No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5990.18255460637996349No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA5750.17524023149996495No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG5670.17280210653996544No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4860.1481160913199704No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4840.14750656007997048No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4580.13958265395997208No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4350.13257304469997347No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4120.1255634354399749No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4010.12221101361997556No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3990.12160148237997567No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3890.1185538261799763No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT3850.11733476369997653No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3710.1130680450199774No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC3630.11062992005997788No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3560.1084965607199783No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3560.1084965607199783No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3480.1060584357599788No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3470.10575367013997886No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC3470.10575367013997886No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3440.10483937327997904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9750.049.2141571
GGGTAAG450.001008352541.815951
AACGCAG11450.039.810466
ATCAACG11850.038.863213
CAACGCA11750.038.7940255
TCAACGC11900.038.3050274
GTACATG19150.036.848261
TACATGG19150.036.563562
ACATGGG19400.035.3656853
GAGCCGA550.002708132134.19749
ACGCAGA13650.033.738397
ATAAGAG702.1835882E-433.566263
AAGGGTA851.7528258E-533.171365
TATCAAC14750.031.8594992
CGCAGAG14550.031.3476168
TAGGCGG600.00415982331.328515
GGTACCT3500.030.8997948
GGGTACC3500.030.8809577
CATGGGG9750.030.846534
ATTATAC804.7778315E-429.3704763
Produced by FastQC (version 0.11.2)