FastQCFastQC Report
Sun 14 Apr 2019
SRR6575738_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575738_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808418
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA11520.14250053808796934No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9540.11800825810409962No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9460.11701867103404426No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8790.10873087932233078No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC8280.10242226175072797No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8230.10180376983194338No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15000.053.4138341
ATCAACG18350.043.7971273
TCAACGC18800.042.7487874
CAACGCA19600.041.4835245
AACGCAG20350.040.1855966
TATCAAC23150.034.9190982
ACGCAGA25500.031.330517
GTACATG36300.030.5110631
TAAGACA5800.029.981914
TACATGG37100.029.8967292
CGCAGAG26950.029.6448238
ACCTAAG4950.029.5156691
GGTAATC1602.1336746E-929.3723538
ACATGGG37700.029.0469253
GTCTTAG3600.028.8015771
GGTATCA6100.028.5869621
GCAGAGT29250.027.4744479
TAGGTAT2600.027.1146165
CTAAGAC6250.025.5672783
GGGTATA2601.8189894E-1225.3776131
Produced by FastQC (version 0.11.2)