FastQCFastQC Report
Sun 14 Apr 2019
SRR6575740_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575740_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences585062
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT28650.4896916907951636No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA27990.4784108350909818No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA10970.1875014955679911No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC10380.17741709425667707No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC10360.17707525014442915No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA9730.16630716060861928No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG9710.16596531649637133No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC9210.157419213690173No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT8940.15280431817482593No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG8830.15092417555746226No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT8610.14716389032273502No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC8490.14511282564924743No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA8320.14220715069514No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA7750.13246459349607392No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG7470.12767877592460286No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC7150.12220927012863594No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG7140.12203834807251196No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC6910.11810714078166074No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA6620.11315040115406573No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC6540.111783024705074No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC6420.10973196003158639No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT6220.10631351890910706No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA6150.10511706451623931No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5950.10169862339375998No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA5860.10016032488864426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTA1305.456968E-1239.7703554
GCATATA651.3828384E-436.285121
TAAGGGT702.1833349E-433.5723724
CTTAGGT1405.0931703E-1033.5723723
TCTTAGG1807.2759576E-1231.3342152
TAGGTAT1551.5152182E-930.3234375
TTAAGGT1102.9645107E-629.9099354
CTATTGA4450.029.571349
AGGTATA1802.382876E-1028.723036
GGCTATT4950.028.4832157
AGGGCTA5050.027.9215775
ATAGAGA856.8109384E-427.6478374
AAGGGTA1205.8354744E-627.417445
CATGGGG17400.027.0122574
GTCCTAC3250.026.1252861
ATAGGGT909.51051E-426.1118453
GGGCTAT5050.026.0601396
CAAGGGT1659.298856E-825.6370854
ACGCAAG1301.0871208E-525.3062428
GCTATTG5400.025.2392968
Produced by FastQC (version 0.11.2)