FastQCFastQC Report
Sun 14 Apr 2019
SRR6575742_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575742_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448200
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA24060.536813922356091No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC15150.33801874163319945No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12700.28335564480142794No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9930.22155287817938418No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7760.17313699241410085No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA7120.15885765283355646No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6640.14814814814814814No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5690.12695225345827754No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5170.11535029004908523No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA4970.1108879964301651No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG4970.1108879964301651No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA4910.10954930834448906No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC4910.10954930834448906No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT4720.10531012940651495No Hit
ACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACGGGAAGGAACTT4520.10084783578759482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15150.040.341811
GTACTAG651.4096909E-436.1644021
AAGCGCG550.00271581734.1803694
GGTAATC2350.033.998558
GTAATCA2650.033.6966749
AAGGGTA2650.031.9231665
CAACGCA19250.031.4947595
ATCAACG19450.031.1709023
AGGGTAA2600.030.7294566
TCAACGC19900.030.7022064
GTACATG30750.030.2722511
AACGCAG20350.030.2542366
CACTATC953.7483187E-529.6829437
CATGGGG14500.029.495294
TATCAAC20850.029.3131142
TACATGG32050.029.0443612
ACATGGG31650.028.956113
ATGGGGG5200.028.921845
GTGCAAG2650.028.3856471
AGGTATA3550.027.8016286
Produced by FastQC (version 0.11.2)