FastQCFastQC Report
Sun 14 Apr 2019
SRR6575742_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575742_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448200
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA25230.5629183400267738No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14450.322400713966979No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC11680.2605979473449353No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC8790.1961178045515395No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG6350.14167782240071397No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG5650.12605979473449352No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA5590.1247211066488175No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5440.1213743864346274No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA5200.11601963409192326No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA5180.11557340473003123No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4880.10887996430165106No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC4810.10731816153502902No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC4720.10531012940651495No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG4690.10464078536367692No Hit
ATCCTATTCCCTACCAAAATGAAGATGCTGTTGCTGCTGTGTTTGGGACT4620.10307898259705489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16500.039.1204951
GGGTACC1750.034.91067
GGTCGTG550.002716654734.1782077
GTTACAC702.1842383E-433.5678833
ACCTAAG8850.033.540151
GTATTAC851.7231032E-533.2583051
CTAAGAC9450.032.821933
TAAGACA9800.032.60884
ATCAACG19900.031.8810543
CAACGCA19800.031.804725
AACGCAG20350.031.4070016
TAGTGCC600.00416044931.3300257
TCAACGC20050.031.1737654
CATGGGG13250.030.8571174
CCTAAGA9950.030.2279622
TATCAAC21500.029.5085092
GGTAATC2250.029.3165761
TGGGTAC2101.8189894E-1229.0921656
GCATATA650.006076626528.994421
AAGACAG11400.028.8566045
Produced by FastQC (version 0.11.2)