FastQCFastQC Report
Sun 14 Apr 2019
SRR6575743_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575743_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416814
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA22970.5510851362958058No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14320.3435585177081384No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC12300.29509565417668315No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9760.2341572020133681No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG7250.17393849534804495No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6530.15666460339623908No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA6450.15474528206826066No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5650.1355520687884764No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC5340.12811469864256No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA4830.11587902517669751No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4750.11395970384871909No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG4720.11323995835072719No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA4640.11132063702274876No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA4640.11132063702274876No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT4340.10412318204282967No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC4190.1005244545528701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGTA2250.035.5044945
CATGGGG12300.032.091584
GTGCAAG2400.031.3425351
GGTAATC2400.031.3274948
GTATCAA17950.030.906011
ATGGGGG4700.028.9945955
GGTACCT1952.0008883E-1128.9176888
AAAACGC650.006155330728.9176865
GTCTTAG4750.028.7031651
GTACATG30250.027.6643221
TGGGTAC2053.6379788E-1127.5070676
GGGTACC2053.6379788E-1127.5070677
TACATGG30800.027.47562
TAGGTAT3950.027.3619885
CAACGCA20900.026.9806165
ATCAACG20950.026.9162243
AACGCAG21300.026.9151736
TGCAAGG3150.026.8650322
ACATGGG30650.026.8302363
CTTAGGT4050.026.6863843
Produced by FastQC (version 0.11.2)