FastQCFastQC Report
Sun 14 Apr 2019
SRR6575743_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575743_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416814
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA24010.5760363135595253No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13810.3313228442422759No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC11320.2715839679089474No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC9120.21880263138954067No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG6150.14754782708834158No Hit
ATCCAATGCCAATCGCTGCCTCCAGGCCCGAGAATGAAGAATGGCCTGAG5690.13651172945246562No Hit
ACTATGTGAGGAGCATGGAATCCTTAGAGAAAATATCATTGACCTATCCA5600.1343524929584899No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA5590.13411257779249258No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5070.1216369891606328No Hit
GTTTGGGACTGACCCTAGTCTGTGTCCATGCAGAAGAAGCTAGTTCTACG4660.11180046735474336No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4610.11060089152475684No Hit
TCACATAGTTGTGCAAACCTTTCCTTGATGTCTGAACTCAAATCTGGTTC4590.11012106119276223No Hit
GTATGGATAGGAAGGGATGATGGTGGAGTCCTGGTGAGAAGTCTCCACTC4450.10676224886879998No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA4420.10604250337080809No Hit
GGATAGGTCAATGATATTTTCTCTAAGGATTCCATGCTCCTCACATAGTT4420.10604250337080809No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA4380.10508284270681888No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4240.10172403038285663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATATG500.001700707737.6003382
GTATCAA13250.035.8783951
CATGGGG12400.035.6250724
ATATTAC550.00271650934.1780246
AATAGTA1900.032.1543245
TTAGGTA3250.031.8118524
AGGTATA3250.031.8118526
TAGGTAT3250.031.8118525
GTACTAG600.004128559531.3787961
TGATCGC600.004143151531.356199
CTTAGGT3300.031.3298553
ACCTAAG8700.030.8377821
GTACATG27800.030.8144281
ACCAGAT8900.030.1230894
GTATAGT3450.029.9928768
ACATGGG27850.029.867423
GGCTATT953.7494417E-529.6809147
TACATGG29100.029.3954522
TGCAAGG2450.028.7757662
TAAGACA10000.028.6668174
Produced by FastQC (version 0.11.2)