FastQCFastQC Report
Sun 14 Apr 2019
SRR6575744_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575744_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865646
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA31540.36435217167294714No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC21100.2437485993119589No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17000.19638512740773945No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11180.12915210143638392No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10910.1260330435304963No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10700.12360710960369482No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9830.11355681190694579No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9610.11101535731696328No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA9330.10778077874789463No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8930.10315995222065372No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8850.10223578691520552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30850.041.7053681
GGTAATC3450.034.0564658
AAGGGTA3600.032.6412165
ATCAACG40450.031.25813
TCAACGC40350.031.2190784
CAACGCA40200.031.2186435
TATCAAC40900.031.1440322
TAGGTAT6350.031.088835
AACGCAG41500.030.6937546
CTTAGGT6750.030.639223
AGGTATA6350.030.348626
TTAGGTA6700.030.166334
ACCTAAG11200.028.6137871
ATGGGAG11650.027.8388965
GTACATG61750.027.2468151
GTAATCA4350.027.0103029
TCTTAGG8200.026.9409452
ACATGGG63200.026.4021973
GTGCAAG4650.026.351471
GTCTTAG8050.026.3451771