FastQCFastQC Report
Sun 14 Apr 2019
SRR6575745_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575745_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792288
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA30570.3858445413788925No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC20900.2637929641746436No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17620.22239387697402965No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA11870.14981925764368514No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC11370.14350842118017693No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG11170.14098408659477363No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11120.14035300294842282No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9190.11599317419928107No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9060.11435235671876894No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8800.11107072175774466No Hit
GTGCAAGGGTAATCATTTATTGAACAGGAAGAGGAAGAAATTCATGAAAA8280.10450745183569611No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8210.10362393473080495No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA8090.10210933397956298No Hit
CATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAGGATGGAATGCA8040.10147825033321217No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7980.10072094995759116No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30650.040.6301571
TCAACGC37550.033.0348474
CAACGCA37900.032.60585
ATCAACG37800.032.567753
AACGCAG38750.031.8905746
AAGGGTA2850.031.3247095
TATCAAC39500.031.2890032
TAGGTAT5100.030.403395
ACCAGAT9550.028.53664694
ACGCAGA43800.028.2136927
AGGTATA5550.027.9382536
CTTAGGT5750.027.7836533
GGTAATC3400.027.6394488
TTAGGTA5650.027.443774
GTCTTAG7200.027.4125791
CGCAGAG45300.027.2794638
GCGCTAT350.00881625126.87009886-87
ACTCGCG350.00884888426.8497578-79
CTACCCC700.008846262526.849754
GTCGACC806.9379894E-826.43022230-31
Produced by FastQC (version 0.11.2)