FastQCFastQC Report
Sun 14 Apr 2019
SRR6575745_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575745_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences792288
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA30120.38016478856173513No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC19790.24978290722565533No Hit
GTCTTAGGTATAGTAAATGTATTGAATCCATCATACGTCACAGAATATTC17240.2175976412617634No Hit
ATCCTAGGTAACATGTGGATAGACTGGAATTGGACTTCTCAGTGAGACAG10510.13265378246294277No Hit
GAATTTATACTGGGGATGCTGTATGGATAGGAAGGGATGATGGTGGAGTC10380.13101296498243062No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA10350.13063431479462015No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA9980.12596429581162405No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9220.11637182438709157No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8980.11334262288460761No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA8560.10804152025526072No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT8360.10551718566985742No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8250.10412880164788563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30500.038.731331
ATCAACG37100.031.4128953
TCAACGC38200.030.754374
CAACGCA37900.030.7498265
AACGCAG39150.029.768036
TATCAAC39950.029.4071852
CTTAGGT6150.029.0361273
ATGGGGG9150.028.7605485
TAGGTAT5950.028.4325435
AGGTATA5850.028.1152786
TTAGGTA6150.027.507914
CATGGGG22450.027.4211184
ACCTAAG11650.027.0668321
GTCTTAG7400.026.7119831
AAGGGTA3000.026.6291875
TCTTAGG7500.026.31592
GTACATG60150.025.9771181
ACGCAGA45300.025.7266757
TAAGACA12650.025.6323724
CGCAGAG45550.025.603258