FastQCFastQC Report
Sun 14 Apr 2019
SRR6575748_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575748_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146368
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4050.27669982509838215No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3440.235024048972453No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3400.23229121119370355No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3190.2179438128552689No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2360.16123742894621776No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2280.15577175338871885No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2240.1530389156099694No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG2200.15030607783121994No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG2160.14757324005247047No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2130.14552361171840839No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2130.14552361171840839No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC2130.14552361171840839No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2030.13869151727153475No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT1990.1359586794927853No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1840.12571053782247485No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG1820.12434411893310013No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1820.12434411893310013No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1790.12229449059903805No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC1770.12092807170966333No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1740.11887844337560123No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1640.1120463489287276No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT1610.1099967205946655No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG1590.10863030170529077No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG1550.10589746392654133No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAACC200.00215938670.498473
ATGGGCG301.3673324E-462.66535
CTCATAC250.005227392556.3987663
GTCGAAG502.9968585E-546.99897494
TCCTATA450.001011162941.776872
TAGGCAT450.001011162941.776875
CTTAGGT450.001011162941.776873
ACCGTCG952.0956577E-839.5780878
ATACCGT1101.7735147E-938.453716
GATCCTA250.001700138137.5991888-89
GTATCAA9450.036.380661
CAGACTC651.407653E-436.153064
CCGTCGT1055.061156E-835.8087439
TACCGTC1055.061156E-835.8087437
TTTAGGG801.1519365E-535.2492333
ATGGGAG1950.033.742855
GACTCTC702.1760145E-433.5706986
AGATAGT851.7446482E-533.1757476
GTGCACT600.004149679631.332654
ACCATGC600.004149679631.332658
Produced by FastQC (version 0.11.2)