FastQCFastQC Report
Sun 14 Apr 2019
SRR6575749_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575749_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378197
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA19000.1378612781772127No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16920.12276909614518099No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG16700.1211728076610238No Hit
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16070.11660161791093727No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14850.10774947268061097No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13910.10092896733921203No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13870.10063873306936527No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19550.053.1388661
ATCAACG25350.040.415763
TCAACGC25300.040.1241154
CAACGCA26450.038.3795855
AACGCAG27850.036.450276
GGTATCA7800.034.3516041
GTACATG52800.032.4956861
TATCAAC33150.031.3383032
TACATGG54350.031.3094752
ACATGGG56250.029.8275723
ACGCAGA34700.029.2547577
GTATAGA1904.474714E-1027.2148441
CGCAGAG38400.026.4359388
CATGGGG35850.024.9078734
ACCTAAG7550.024.9046121
TAGGTAT4550.024.7897435
GCAGAGT42800.023.937849
GTCTTAG6800.023.5037291
AGGTATA4600.023.4986116
TTAGGTA4700.022.998644
Produced by FastQC (version 0.11.2)