FastQCFastQC Report
Sun 14 Apr 2019
SRR6575749_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575749_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1378197
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA15510.11253833813308256No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15390.11166763532354228No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT15340.11130484248623382No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15270.11079693251400198No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA14060.10201734585113739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19400.053.2110561
TCAACGC26250.037.962164
ATCAACG26550.037.5345653
CAACGCA27500.035.723825
AACGCAG28800.034.437716
GTACATG52950.031.0684031
TACATGG53250.030.8964422
TATCAAC34500.029.7028032
ACATGGG56150.029.2170093
ACGCAGA34800.028.6290137
AGGTATA4150.027.1836286
ACCTAAG6300.026.9352761
GGTATCA8100.026.1870751
CGCAGAG38600.026.1758548
CTATTAA3450.024.593081
TAGGTAT4900.023.9821575
TTAGGTA5300.023.9459554
CATGGGG34300.023.7080734
GGTACCT7850.023.3477338
GCAGAGT42800.023.1679959
Produced by FastQC (version 0.11.2)