FastQCFastQC Report
Sun 14 Apr 2019
SRR6575751_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575751_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313305
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA16540.12594180331301563No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA15730.11977415756431294No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG14670.1117029174487267No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT14530.1106369046032719No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC14070.10713429096820618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18350.054.066351
TCAACGC26350.036.920874
ATCAACG26700.036.0862163
CAACGCA27200.035.4215165
AACGCAG27950.034.639186
GTACATG52800.032.1481
TACATGG53400.031.7867872
ACATGGG55900.030.2683983
TATCAAC34650.028.3611432
ACGCAGA37100.026.0960947
ACCTAAG6150.025.9946781
TAGGTAT5100.025.8029925
CATGGGG36400.025.306784
CGCAGAG38900.024.8885658
CTTAGGT5500.024.781873
CTAAGAC8950.024.1564733
AGGTATA5300.023.9425336
ATGGGAG14100.023.6658135
AGAGTAC36500.023.3060110-11
GTATAAT5500.023.0824411
Produced by FastQC (version 0.11.2)