FastQCFastQC Report
Sun 14 Apr 2019
SRR6575752_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6575752_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167549
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAAGACAGACTATGATAACTTTCTTATGGCTCATCTCATTAACGAAA14680.12573348099308895No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13720.11751112801261447No Hit
CTATTATCCTGGCCTCTGACAAAAGAGAAAAGATAGAAGATAATGGCAAC13680.11716852997176136No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13230.11331430201216394No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12720.10894617699128688No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17200.050.2752881
ATCAACG22850.037.0149463
TCAACGC23000.036.5692444
AACGCAG24100.035.288546
CAACGCA24100.035.093585
GTACATG44400.031.8602161
TACATGG45450.031.0207712
ACATGGG45800.030.4706543
TATCAAC28800.029.2096122
ACCTAAG5500.029.0523621
TAAGACA9150.027.7308674
CTAAGAC8150.027.6741583
GTACTAT1704.0799932E-927.644981
GGTATCA7150.026.9490221
ACGCAGA32400.026.2485777
CATGGGG27300.025.645694
CGCAGAG33850.025.1241918
GTATAAT4250.024.3275831
AGAGTAC31300.022.6675610-11
GCAGAGT38500.022.089719
Produced by FastQC (version 0.11.2)